THE ACTIVITY OF CARBONIC ANHYDRASE IN PATIENTS WITH

THE ACTIVITY OF CARBONIC ANHYDRASE IN PATIENTS WITH

ZOLLINGER-ELLISON SYNDROME.ADJUVANT DIAGNOSIS TEST.

Zollinger-Ellison syndrome represents a peptic ulceration with gastric hypersecretion and non-beta cell tumor of the pancreatic islets, sometimes associated with familial polyendocrine adenomatosis.

AIM: a follow-up of carbonic anhydrase (CA) activity in red cells and parietal cells (PCs) isolated f 232d36c rom human gastric mucosa, purified CA I and CA II activity after association with serum of Zollinger-Ellison (ZE) patients.

METHOD: In a group of Zollinger-Ellison patients (N=7) and a control group (N=8) we followed CA activity in isolated parietal cells and red cells, as well as CA activation by separate association of histamine (Hs) and pentagastrin (P) in concentrations of 10^-8-10^-4 M according to dose-response relationship. We also followed the effect of Zollinger-Ellison patients serum, in dilution between 1/1000 and 1/100 on purified CA I and CA II as well as on isolated parietal cell CA. CA activity was determined by stopped-flow method (Khalifah, 1971).

RESULTS:

CONCLUSIONS: Red cell and parietal cell CA activity is incresed by over 100% in patients with Zollinger-Ellison as compared to controls. Histamine and pentagastrin slightely activate CA in Zollinger-Ellison patients as compared to controls. The serum of these patients activates CA II isozyme by over 250% as compared to the serum of controls. These results show that in Zollinger-Ellison patients exists a higher CA activity in red cell and in parietal cells as well as the presence of activating effect of the serum. Our data suggest that these chnages could be induced by hypergastrinemia, as well as by releasing of endogenous Hs induced by gastrin. This method could be used as on additional test in the diagnosis of Zollinger-Ellison syndrome.